Wales midfielder Aaron Ramsey will miss the opening Euro 2020 qualifier against Slovakia on Sunday after failing to recover from injury.
The 28-year-old has been withdrawn from the squad and has returned to Arsenal for treatment.
Ramsey did not feature in Wales' 1-0 friendly win against Trinidad and Tobago on Wednesday.
"We have plan B and we've worked on it during the week," said Wales manager Ryan Giggs.
"He was in good form but it gives a chance for someone else."
Ramsey's absence could open up an opportunity for Matt Smith, Lee Evans or Will Vaulks to play a key role in Sunday's match.
Ryan Giggs was without Ethan Ampadu, Sam Vokes and Tom Lawrence for Wednesday's friendly, while David Brooks also missed out last minute with an ankle knock.
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Fatherof
55
Swear to God after the next international break I wanna see a statement/announcement when it's over and the players return back to their clubs saying 'No Arsenal players were harmed during this process'
nutmegprince
54
Now I can see the relationship btw Bale and Ramsey