The striker was injured in a clash with Fabian Delph, and was unable to put any pressure on his ankle after the challenge
Tottenham manager Mauricio Pochettino has admitted that striker Harry Kane could be out for the rest of the season.
Kane had to be removed from Tuesday's Champions League match against Manchester City with a left ankle injury.
In the 55th minute, the Spurs star attempted to block a clearance by Fabian Delph and the City defender inadvertently stepped on Kane's ankle as he was following through.
Kane immediately had to be helped down the tunnel, unable to put any pressure on his troublesome left ankle.
The Tottenham star was replaced by Lucas Moura, with the Brazilian coming off the bench in the 58th minute of a scoreless quarter-final first leg.
Spurs went on to win the match 1-0, getting a 78th-minute goal from Son Heung-Min to give them the advantage ahead of the second leg.
Kane's injury will be a major worry for Tottenham and for England, as the striker injured the same left ankle that he hurt back in January.
The striker was out for six weeks after suffering torn ankle ligaments in a loss to Manchester United on January 13.
With the calendar having shifted to April, there will be real fears that Kane may not play again for Spurs this season.
Pochettino, speaking about Kane's injury to BT Sport said: "It's very, very sad. It's going to be tough and we're going to miss him - maybe for the rest of the season."
Spurs have six Premier League matches remaining, with Mauricio Pochettino's side holding onto the fourth and final Champions League spot, one point ahead of fifth-placed Arsenal.
Kane will likely also miss Tottenham's return leg against Man City, which will take place next Thursday at the Etihad Stadium.
The 25-year-old is in the middle of another strong campaign, having scored 24 goals in 38 appearances in all competitions for Spurs in 2018-19.
Kane's status may also be in doubt for England's UEFA Nations League semi-final against the Netherlands on June 6.
Beemstz
144
Too bad for him. I expected a competitive PL topscorer with Kane in but it's rather unfortunate that he could be out. Wish you a speedy recovery Harry
JuniorGunner•••
98
The sun (son) shines in the absence of Hurricane ( Harry Kane) IYKWIM
poebcilyz
87
why not a Red CARD what happen to VARRRRRRRRRRRRRR
Elsa98
70
A huge blow for spurs!😢 Please get well soon, kane.🙏🏻
losdilmnu
55
Everyone saying where was VAR it was not intentional. They both went for the ball, Delph can't just remove his leg or let Kane take the ball, that's not how football works. They both fought for the ball, shame to see Kanes season come to an end but that's football sometimes. Hope he recovers quickly, but it seems like he is missing the rest of the season. There was not really an issue with the refs aside from a couple pulls between the teams. Everything else was just the game being as intense as we expected it to be.
SWEEPERKEEPER
8
why not a Red CARD what happen to VARRRRRRRRRRRRRR
That was foul play by Kane.