The 31-year-old forward has decided to bring his international duty to an end after having played 72 matches and scored 31 goals. He will no longer play for Argentina.
After giving a statement to 'Fox Sports', the Chelsea player has confirmed that he has reached the decision that it would be best to leave a space for the younger generation to come through in the Argentina squad.
"My relationship with the national squad is normal. I want to leave it in the best way, and I believe that I have", confirmed the Argentine forward.
"I want to enjoy my family, and my time. To the joy of many, and maybe not so much for others, my time is up.
"You can now stop worrying about whether I am there or not."
Higuain & Argentina National Team
Higuain has endured a fractious relationship with the national team in recent years, with costly mistakes in the 2014 World Cup final and 2015 Copa America decider constantly used to question his talents.
His last goal for Argentina came in October 2016, in a World Cup qualifying draw against Peru, and he failed to find the net in Russia as the Albiceleste crashed out in the last 16 to eventual winners France.
The striker made his international debut in October 2009 under Diego Maradona, instantly opening his account in an agonising 2-1 win over Peru that sealed Argentina's place in the World Cup the following year.
In South Africa he then became the first Argentina player to score a World Cup hat-trick since Gabriel Batistuta with his triple against South Korea, and scored again in a 3-1 win over Mexico in the last 16.
Higuain finished his international career with a total of 31 goals in 75 games, a tally that puts him in sixth place in the Albiceleste's all-time standings behind Maradona.