Luke Shaw is set to return to Manchester United's medical team on Monday after leaving the England squad having suffered concussion in the Nations League defeat to Spain.
Shaw had to be substituted early in the second half at Wembley following a collision with Spain's Dani Carvajal and stayed at St George's Park on Sunday to be monitored.
However, the left-back will now return to United for further assessment, with Leicester's Ben Chilwell having been drafted into the squad to replace him, while winger Demarai Gray has also been called up.
A statement from the Football Association read: 'Luke Shaw remained at St. George's Park today for further rest and monitoring having sustained concussion against Spain yesterday. He will return to his club tomorrow.'
It seems likely that Shaw will miss United's trip to face Watford on Saturday with the Premier League having strict concussions guidelines which dictate that players cannot train until receiving clearance.
This setback represents a further setback for Shaw, who seemed to have finally got his career back on track having endured many difficulties after suffering a double leg break while playing for United against PSV in 2015.
Shaw had started United's first four games of the season, which included him scoring against Leicester in the opening fixture, and he was named in the starting lineup against Spain.
However, Shaw now faces another spell on the sidelines, which means that Ashley Young, who wasn't included in the England squad, could start at left-back against his former club Watford.