Ruben Amorim is taking a 26-man squad to Bilbao for Wednesday's UEFA Europa League final against Tottenham Hotspur.
Included in it is Diogo Dalot, Leny Yoro and Joshua Zirkzee, who all took part in Tuesday morning's training session at Carrington following their respective recent absences.
Matthijs , was spotted going through an individual workout on another pitch and is one of only two first-team players to miss out through injury, with long-term absentee Lisandro Martinez being the other.
Young forward Chido Obi is ineligible for the Europa League.
Travelling Squad
Goalkeepers
Altay Bayindir
Tom Heaton
Dermot Mee
Andre Onana
Defenders
Harry Amass
Patrick Chinazaekpere Dorgu
Diogo Dalot
Jonny Evans
Tyler Fredricson
Ayden Heaven
Victor Lindelof
Harry Maguire
Noussair Mazraoui
Luke Shaw
Leny Yoro
Midfielders
Casemiro
Toby Collyer
Christian Eriksen
Bruno Fernandes
Kobbie Mainoo
Mason Mount
Manuel Ugarte
Forwards
Amad
Alejandro Garnacho
Rasmus Hojlund
Joshua Zirkzee