A minute's silence held in memory of the Queen was cut to 25 seconds last night after a minority of Liverpool fans began to boo and chant as soon as it happened.
Many supporters at Anfield tried to shush and drown out the noise from those who chose to yell rather than observe the tribute to Britain's longest-reigning monarch.
The incident took place before the club's Champions League game against Ajax. Liverpool has a history of anti-royalist sentiment among some supporters, which has included booing the national anthem and Prince William in the past.
After Her Majesty's death and King Charles III becoming monarch, the club made a request to UEFA for the tribute to be held following the late monarch's death on Thursday, with manager Jurgen Klopp insisting it was the right thing for the club to do in this momentous period. The Hillsborough Survivors group also urged fans to show 'respect'.
As the 53,000-strong crowd stood for a moment of reflection ahead of the Group A fixture, there were isolated boos, shouts and chants of 'Liverpool, Liverpool' from a minority of individuals.
The referee then blew the whistle to start the match after just 25 seconds.
There is antipathy towards the monarchy in some quarters of Merseyside and there was a furore before the FA Cup final in May when Prince William, the new Prince of Wales, was booed by Liverpool fans.
Sections of Liverpool supporters also booed the national anthem, as had been the case before the Carabao Cup final in February, while others sang along at Wembley.
The same occurred prior to the Community Shield victory over Manchester City at Leicester's King Power Stadium in July, where Prince William was also jeered as he shook the hands of the players.
Consequently, all the attention has been on Liverpool fans at Anfield and whether they would show their respects to the longest-reigning monarch in the country's history.
The tribute was held just before the match kicked off at 8pm tonight, with minimal noise or disruption from the crowds. Although, as BT Sport's Darren Fletcher said, 'Impeccably observed by many, but not by everyone.'
The players wore black armbands for the match with the traditional pre-match Champions League anthem not played.
The game against the Dutch team is Liverpool's first Champions League home match of the 2022/23 season, with pressure already mounting after the club's defeat against Napoli in Naples last week.
Earlier today, the Hillsborough Survivors Support Alliance warned Reds supporters that they may have faced increasing abuse over Hillsborough should they have decided to boo the anthem or the minute's silence in memory of Her Majesty.
The group posted: 'Everyone attending tonight's match, please show respect and observe the minutes silence.
'You probably think that any backlash won't affect you but it will affect us and family members with the increase in abuse over Hillsborough.
'Respect costs nothing and that goes both ways.'