Andrew Robertson, Scott McTominay and Ryan Fraser have withdrawn from the Scotland squad for the European Qualifiers against Cyprus and Kazazkhstan through injury.
They join Liam Cooper on the sidelines for the matches in Nicosia on Saturday and Glasgow on Tuesday.
Graeme Shinnie and Lewis Morgan have been called up as replacements.
Robertson played the full 90 minutes in Liverpool's 3-1 win over Manchester City on Sunday while Fraser also completed the match as Bournemouth lost 2-1 at Newcastle.
McTominay injured his ankle in Manchester United's 3-1 win over Brighton on Sunday and was substituted shortly before the end of the game at Old Trafford.
Scotland are already missing Kieran Tierney after Arsenal requested he be left out of the squad.
Scotland cannot qualify from Group I but can progress to their first major tournament since 1998 via the Nations League play-offs, which take place in March.