Barcelona coach Ernesto Valverde included youngsters Alena, Miranda, Inaki Pena and Chumi in his squad to face Villarreal on Sunday.
Arthur continues to have problems with a thigh strain that saw him miss training on Saturday and he misses the game.
Luis Suarez misses out on squad as well with knee injury.
Barcelona squad: Ter Stegen, Semedo, Pique, Rakitic, Busquets, Denis Suarez, Coutinho, Messi, Dembele, Malcom, Lenglet, Alba, Munir, Vidal, Alena, Miranda, Inaki Pena and Chumi.