The Football Association has condemned those responsible for the disorder in Seville on Sunday night ahead of England's Nations League match against Spain.
Supporters had to be dispersed by riot police as Gareth Southgate's side prepare for Monday night's match.
Video footage shows noisy supporters kicking wing mirrors off parked vehicles, aggressively circling motorists and one man throwing a glass at a passing car in central Seville.
Their shocking behaviour follows pleas by British police to travelling fans to avoid a repeat of the scenes that followed the England football team's last away game in Spain three years ago.
A statement from the FA released on Monday afternoon read: 'We condemn the unacceptable behaviour witnessed in Seville.
'With the Spain fixture still to come on Monday night, we urge those following the team to respect our hosts and act in a responsible manner.
'The FA will work in partnership with the UK Football Policing Unit to help identify those involved and we will sanction any England Supporters' Travel Club member found to be responsible for disorder.'
Around 3,000 fans have travelled to the southern Spanish city, and England supporters were out in force on Sunday night ahead of Monday's game.
Riot police had to intervene to fire warning shots in the air as the situation threatened to spiral out of control, provoking a mini-stampede among some fans.
When similar trouble occurred surrounding the friendly against Holland in Amsterdam in March, 13 members of the ESTC were identified as being among the trouble-makers.
It is understood one member of the ESTC, who was caught on camera throwing unattended pedal cycles into a canal, was given a life ban for his misconduct in Amsterdam.
A further 10 were given three-year bans while a further member remains suspended.