Guardiola was furious with substitute John Stones for not being ready to come on in the first-half against Atalanta on Tuesday.
Midfielder Rodri, who was playing at centre back, seemed to injure his hamstring in the first half and hobbled off the pitch.
But much to Guardiola's frustration, Stones was not prepared at all to come on, and the City boss was visibly upset at the lack of urgency from the England international.
As Stones sorted out his shin pads and socks, Guardiola walked up the steps and motioned to the defender if he was ready or not.
He then slammed the chair and walked hurriedly down the steps, as Stones eventually sorted himself out and came on to replace the stricken Rodri.
City were winning the match 2-1 at the time but Guardiola could not hide his anger at the situation that unfolded.
Furthermore, the injury to Rodri gives the Manchester City manager another defensive headache.