Ciro Immobile, Giorgio Chiellini, Lorenzo Insigne, Jorginho and Alessandro Florenzi will not take part in Italy’s friendly with the USA.
The Azzurri were held to a 0-0 draw by Portugal on Saturday evening, which closes their Nations League experience.
There is a friendly against the United States to be played in the Belgian city of Genk on Tuesday evening.
Coach Roberto Mancini has cut several players from the squad going into that game, so he can focus on bringing through younger and less experienced figures.
It was already confirmed that Chiellini, Florenzi, Insigne and Jorginho would be allowed to go back to their clubs early.
Now Lazio striker Immobile has also been cleared to leave the Nazionale training camp.