Spain coach Luis Enrique dropped David de Gea and included Brentford goalkeeper David Raya on Friday in the squad for upcoming friendly matches against Albania and Iceland.
De Gea played the entirety of Manchester United's 1-0 defeat against Atletico Madrid on Tuesday night, a result which dumped them out of the Champions League at the last-16 stage.
He was Spain's starter at a disappointing 2018 World Cup, where they crashed out in the round of 16, before becoming backup to Unai Simon, who helped them reach the semi-finals of the European Championship last year.
The 26-year-old Raya was born in Barcelona but has played in England for several years. He was at Blackburn before joining Brentford in 2019.
Raya will likely be third-choice goalkeeper behind Athletic Bilbao's Simon and Robert Sanchez of Brighton.
Spain will host Albania in Barcelona on March 26 and Iceland in A Coruna on March 29 as it starts preparation for the World Cup in Qatar.
De Gea's omission comes as a surprise given his solid form for United this season, which recently saw him claim their Player of the Month prize in January.
He has made the second-highest number of Premier League saves in 2021/22, with his tally of 105 only bettered by Leeds shot-stopper Illan Meslier (111).
Earlier this year, United interim boss Ralf Rangnick insisted De Gea was one of the top five goalkeepers in world football while comparing him to Bayern Munich No 1 Manuel Neuer, whom he managed at Schalke.
'Well, I think they are both definitely on the list of the top five goalkeepers in the world, maybe even in the top three,' Rangnick said.
'They are both in a fantastic shape, physically and as well as on the line, when it's about controlling the box, David de Gea is probably one of the best if not the best in the world and we are very happy to have him.'