Roma fans unveiled an X-rated banner slamming Anthony Taylor during their 2-1 win against Spezia on Sunday night.
It comes as videos have emerged online of Italian club's fans harassing the English referee after they were beaten by Sevilla in the Europa League final on Thursday night.
Taylor, who officiated the game, was criticised by the Serie A side's boss Jose Mourinho in the car park after the match, calling him a 'f****** disgrace'.
The Italian manager has since been charged by UEFA for his actions and slammed by many throughout the football community, while the 44-year-old has also received abuse from Roma supporters, who ambushed him and his wife at Budapest airport.
A video clip surfaced online of the unsettling scenes, showing individuals hurling abuse at the referee, after he and his wife had to be ushered to safety.
But it appears he has continued to receive abuse from Roma fans, after they unveiled a banner tonight inside the Stadio Olympico that read: 'Taylor f****** shame'.
The Italian side were beaten 4-1 on penalties on Thursday night, with Mourinho becoming incensed over several decisions during the match.
His side were not given a penalty for a handball, while Taylor showed eight yellow cards to Roma during the game.
Taylor also ordered a re-take of the decisive penalty that won Sevilla the title after the Roma goalkeeper had saved the spot-kick.
After arriving home following the final, the former prison guard was today seen walking his dog with his wife in his home of Altrincham, Greater Manchester.
Many have come to the support of Taylor, with the Premier League condemning the 'unnaceptable abuse' directed towards the official after the match.
Meanwhile, a CEO of a refereeing charity has blamed Mourinho for the abuse he has received.
Rio Ferdinand has called for the 'thugs' that hurled abuse at the referee 'should be held accountable' while Jeff Stelling echoed his sentiment, condemning their actions.
He said: 'Jose Mourinho's actions and words contributed massively to the disgraceful behaviour towards Anthony Taylor. He is a repeat, repeat, repeat offender.
'Needs the strongest possible punishment.'