According to the Daily Mail, Saka is unlikely to start against Ghana, while Rashford is currently dealing with a minor hip injury.
Saka is unlikely to start in England's next match against Ghana, and Rashford is also dealing with a minor injury.
Rashford came off the bench in England's 4-2 victory over Croatia on Wednesday and impressed by scoring the team's final goal.
However, he experienced hip discomfort and did not participate in England's 5-1 win over Sporting Kansas City in a closed warm-up match on Thursday.
The injury is understood to be minor, but it could mean Anthony Gordon will continue as left winger in next Tuesday's match in Boston.
Tuchel values players like Rashford who can impact the game from the bench, particularly as opponents tire, so the coach may prefer his impact role over calls for him to start.
Kane left AT&T Stadium with his left calf bandaged, but it is understood this was only due to cramps. He will be available for the second group stage match.
Tuchel is not expected to start Saka until the final group stage match at the earliest. Saka provided the assist for Rashford's goal in Dallas but was absent from training the following day.
Saka is gradually regaining fitness after being troubled by an Achilles issue, so Madueke is expected to continue at right wing.
Ivan Toney scored another hat-trick against Sporting Kansas City, adding to his hat-trick against Miami FC a week prior. Morgan Rogers and Watkins scored the other two goals.
Marc Guehi also featured in the match. After Tuchel paired Konsa with Stones at center-back against Croatia, Guehi is fighting to regain his starting position.